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KMID : 1134820090380030359
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Journal of the Korean Society of Food Science and Nutrition
2009 Volume.38 No. 3 p.359 ~ p.363
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Determination of Niacin in Infant Formula by Solid-phase Clean-up and HPLC with Photodiode Array Detector
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Hong Jee-Eun
Kim Ok-Hee
Park Eun-Ryong
Mun Chun-Sun
Lee Kwang-Ho
Gwak In-Shin
Kim Mi-Ran
Cheon Sang-Hee
Chai Jung-Young
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Abstract
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This study was performed to establish a rapid and simple analytical method for niacin (nicotinic acid and nicotinamide) using HPLC. A pretreatment method for the extraction and clean-up of niacin in infant formula sample and an instrumental condition for HPLC were optimized. Niacin was extracted by 5 mM hexanesulfonate with ultrasonication for 30 min. For the clean-up of the sample, the extract was applied to a HLB cartridge, and then niacin was eluted from the cartridge using 5 mL of 80% methanol after washing with 5 mL of n-hexane. The total recoveries were 83～104% and relative standard deviation were in the range of 1.5～3.5% during the extraction and clean-up process. Niacin was determined by gradient elution with sodium hexanesulfonate/methanol buffer as a mobile phase and a photodiode array detector (260 ㎚). It showed a high linearity between the content of niacin and the peak area (r²=1.000) in the range of 0.02～10.0 ㎎/L of nicotinic acid and nicotinamide. The detection limit was 0.02 ㎎/L (0.2 ㎎/㎏ in the sample). The method was successfully applied for the determination of niacin in infant formula. Total niacin contents were in the range of 53.5～140.3 ㎎/㎏.
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KEYWORD
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niacin, HPLC, solid phase extraction, infant formula
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